<p>The oral LD<sub>50</sub> in rats is 135mg/kg and in mice is 146mg/kg.<sup class="text-reference-group"><a class="reference-popover-link" data-content="Cayman Chemicals: Methotrexate MSDS" href="#reference-L7204">9</a></sup></p><p>Symptoms of overdose include hematologic and gastrointestinal reactions like leukopenia, thombocytopenia, anemia, pancytopenia, bone marrow suppression, mucositis, stomatitis, oral ulceration, nausea, vomiting, gastrointestinal ulceration, and gastrointestinal bleeding.<sup class="text-reference-group"><a class="reference-popover-link" data-content="FDA Approved Drug Products: Methotrexate Injection" href="#reference-L7180">7</a></sup> In the event of an overdose, patients should be treated with glucarpidase and not be given leucovorin for 2 hours before or after glucarpidase.<sup class="text-reference-group"><a class="reference-popover-link" data-content="FDA Approved Drug Products: Methotrexate Injection" href="#reference-L7180">7</a></sup></p>